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Catalog# ProductDescription |
ICP1001 Protein A binds to the immunoglobulin G and M of many mammalian species. The product is protein A chemically linked to beaded agarose through a stable covalent linkage. The immobilized protein A on the beaded agarose could be utilized for the immunoprecipitation and purification of both polyclonal and monoclonal antibodies from mammalian species.
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Formulation |
10 mg; 1 mL Agarose Beads suspended in 2 ml slurry in glycerol
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| Ligand Immobilized |
10 mg protein A/mL matrix. The matrix linking chemistry is stable when eluted with weak acid (pH 2-3) and could be re-usable with no apparent loss of IgG binding capacity.
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| Binding Capacity |
30-40 mg rabbit IgG/mL beads as tested using rabbit serum
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| Applications |
IP, IAC
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| Scientific Description |
Protein A agarose consists of the native protein A immobilized on 4% cross linked agarose beads. It is made for the binding of immunoglobulins. Protein A is very heat and acid stable.
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| Storage & Stability |
Product is stable for 30 days at ambient temperatureFor extended storage store product at 4 to–20oC.
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| Product Specific References | 1. Trends Biochem. Sci. 1982. 7: 74-76. doi:10.1016/0968-0004(82)90082-22. 2.Rowan University School of Osteopathic Medicine. 2019. YfmK is a Novel Ne-lysine Acetyltransferase that Directly Acetylates the Histone-like Protein HBsu in Bacilius subtilis | ||
