掃一掃，添加微信號(hào)
Acetyl Lysine Antibody, FITC
|
Catalog# Product Description |
ICP0385 The affinity purified rabbit polyclonal anti-acetylated lysine antibody was developed using a unique technique that utilizes acetylated KLH as the immunogen and acetylated lysine-specific antibodies. It was specifically purified with immobilized acetylated lysine on agarose. The purified antibody is conjugated to fluorescein isothiocyanate. |
|
Immunoflourescent staining of the TSA-treated and non-treated melanoma cells.
|
|
Species
|
Rabbit
|
||
|
Formulation
|
250 μg/mL antibody in PBS, pH 7, with 50% glycerol
|
||
|
Immunogen
|
Acetylated KLH conjugates
|
||
|
Purification
|
The antibody was purified on acetyl-lysine agarose.
|
||
|
Conjugation
|
FITC/Ab ratio - 10:1, followed by G25 filtration
|
||
|
Applications
|
For direct immunofluorescence experiments
|
||
|
Scientific Description
|
Acetylation of lysine is an important, reversible modification. The activities of some proteins are controlled by acetylation of lysine. Histone acetyltransferases (HATs) acetylate the conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) that contain lysine residues. Histone deacetylases (HDACs) remove the acetyl group from the same residue. [1]Acetylation/deacetylation of histones results in cell signalling processes that include gene activity, cell growth, differentiation and apoptosis. [2] In cancer and polyglutamine diseases, the regulation of protein acetylation/deacetylation is impaired (7). Numerous anti-cancer drugs target HDACs. [3]
|
||
|
Storage & Stability
|
Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is six months from date of shipping if stored properly.
|
||
|
Product Specific References |
|
